Commit 455bd1a7 authored by Turnhout, M.C. van's avatar Turnhout, M.C. van
Browse files

per channel analysis now possible

parent ffab9d30
......@@ -12,6 +12,8 @@ Many thanks for the critical review of v0.02 by Ayla Hokke, Lars Houben, and Nik
* [fix] proper handling of existing DMAlab_param-files when selecting a target with uigetfile
* [fix] proper handling of new FoV selection in DMA_setfluoparam.m
* [fix] restore cell size check and bwg-functionality in DMA_countfluo.m
* [feature] set fluorescence cell detection parameters per channel
* [feature] add contrast stretching for fluorescence analysis
* [feature] open dialogue box in DMA_setfluoparam.m for missing droplet data
* [feature] copy new settings in DMA_labconfig to existing DMAlab_param-files to improve backward compatibility (DMA_checkparam.m)
* [update] split droplet detection from DMA_analyseFoV.m into dedicated script DMA_analyseBF.m
......
......@@ -85,11 +85,11 @@ for t = 1:nframe/param.nchan
% perform fluorescence analysis
if param.cellminIraw > 0
[raw, ~, BW] = DMA_preprocessfluo(F, mask, ch, param);
[raw, ~, BW] = DMA_preprocessfluo(F, mask, c, param);
else
[~, raw, BW] = DMA_preprocessfluo(F, mask, ch, param);
[~, raw, BW] = DMA_preprocessfluo(F, mask, c, param);
end
[count, mi] = DMA_countfluo(raw, BW, param);
[count, mi] = DMA_countfluo(raw, BW, c, param);
% store data
ncel(n, c+numel(param.fluo)*(t-1)) = count(1); % number of detected cells
......
% written by DMA_writeparam
% https://gitlab.tue.nl/STEM/DMAlab
% @ 25-Mar-2020 17:26:06
% @ 01-Apr-2020 19:44:20
param.dir = '/home/mark/git/DMAlab/data';
param.nbit = 14;
param.BF = 1;
......@@ -19,6 +19,9 @@ param.cellminA = [10, 10, 10];
param.cellmaxA = [1000, 1000, 1000];
param.celltophat = [8, 8, 8];
param.cellthresh = [-1.000000, -1.000000, -1.000000];
param.cellstretch = [0.010000, 0.300000;
0.010000, 0.300000;
0.010000, 0.300000];
param.maxgapn = 2;
param.maxgapw = 2;
param.iscale = 100;
......
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